It is there, but in some cases, we use other tools like models to replace. As for [url="http://www.creative-animodel.com/Animal-Model-Development/Conditional-Knockout.html"]conditional models[/url] we choose for different disease conditions.

Where did you do your internship? What did you do? I have heard that CRISPR/Cas9 System is a very famous tool for genome editing, it must be a great honor to do that with internship experience!

In the drug research and development, we need many tools to realize the optimal drug preparation for disease treatment. Here are two transgenic mice preparation methods for you:
Pronuclear microinjection
ES cell based gene overexpression

Method of emergency rescue after drowning：
The most important thing is to immediately carry out artificial respiration and chest compression.