₦airaland Forum

Increase In Absorbance For Substrate And Blank (Carbonate Bicorbonate)

Procedure: 0.1ml of the serum was added to the tubes containing 0.75ml of ethanol and 0.15ml of chloroform under chilled condition and centrifuged. To 0.5ml of supernatant, 0.5ml of 0.6mM EDTA solution and 1.0ml of 0.1M carbonate-bicorbonate (pH 10.2) were added. The reaction was initiated by the addition of 0.5ml of 1.8mM epinephrine and the increase in absorbance at 30 seconds interval for 3 minutes was measured at 480nm in a Shimadu UV spectrophotometer.

Calculation:
Increase in absorbance for substrate = A3 – A0
2.5
where
A3 = Absorbance after 150 secs
A0 = Absorbance after 30 secs.
% inhibition
= 100 – {(increase in absorbance for substrate)/(increase in absorbance for blank)} x 100/1
One unit of SOD activity was given as the amount of SOD activity to cause 50% inhibition of the oxidation of adrenaline.
SOD activity = (% inhibition)/50
Unit is in I.U.

3.2.6.2 Catalase Activity
Method: Calatase was aasayed according to the method of Takahara et al. (1960).
Principle:
In the UV range, H2O2 shows a continued increase in absorbance with decreasing wavelength. The decomposition of H2O2 can be followed directly by decrease in extinction at 240nm. The difference in extinctions, DE240 per unit time is a measure of the activity of catalase.
Reaction
2H2O2 2H2O + O2

Procedure:
To 1.2ml of 0.01mM phosphate buffer (pH 7.0), 0.5ml of serum was added. The enzyme reaction was started by the addition of 1.0ml of 0.2mM hydrogen peroxide solution. The decrease in absorbance was measured at 240nm for every 30 seconds up to 3 minutes. the enzyme blank was run simultaneously with 1.0ml of distilled water instead of H2O2.

Calculation:
K = 2.303/∆t x (Log E_1)/E_2
where
t = Change in time
E1 = Absorbance at t6 i.e 180 sec
E2 = Absorbance at t1 i.e 30 secs.
K = Reaction rate.
Unit is iu/mg of protein.

Glutathione peroxidase (GPX) Activity
Method: The Rotouck et al. (1973) method was used.
Principle:
The increase in absorbance of the solution was measured when 1.0ml of dithionitrobenzene (DTNB) was added and read at 420nm immediately.

Procedure:

The reaction mixture consisting of 0.2ml of 0.8mM EDTA, 0.1ml of 10mM sodium azide, 0.1ml of 2.5mM H2O2, 0.2ml of GSH, 0.4ml of 0.4mM phosphate buffer (pH 7.0) and 0.2ml of serum was incubated at 370C for 10 minutes. The reaction was arrested by the addition of 0.5ml of 10% TCA and the tubes were centrifuged at 2000 rpm. To the supernatant, 3.0ml of 0.3m disodium hydrogen phosphate and 1.0ml of DTNB were added and the colour developed was read at 420nm immediately. The activity of glutathione peroxidase was expressed as µmoles of glutathione oxidized/minutes/mg of protein.
Calculation:
GPX activity = (Absorbance of the sample/minute)/(0.840 X 10)
where
0.840 = Absorbance of the standard
10 = Absorbance of the standard

Glutathione-s-Transferase (GSTs) Assay
Method: GSTs was assayed by the method of Habig et al. (1974).
http://www.prestarticles.com/increase-in-absorbance-for-substrate-and-blank-carbonate-bicorbonate/

Lil Kesh - People Still Don’t Get It
Few weeks ago a rumour that a YBNL act was leaving the group filtered into the internet, both Lil kesh and his boss Olamide has confirmed the rumour to be true.
http://www.prestnaija.com/lil-kesh-people-still-dont-get/

Sunday Message - Unveiling The Unlimited Power Of Faith
Let’s talk about faith in this week’s episode of Sunday Message, Faith as it were is the hope of having a thing that you have not seen, now let’s make this Sunday message exciting while you read on.
http://www.prestnaija.com/sunday-message-unveiling-unlimited-power-faith/

SHOCKING - Tiwa Savage Actually Slept With 2face Once

Just like every other person, I am actually tired of the Teebillz and Tiwa Savage saga which has continued to stay relevant in the industry.
http://www.prestnaija.com/shocking-tiwa-savage-actually-slept-2face/

Yaya Toure signs for A French Outfit

Young Yaya Toure has successfully negotiated a deal and has signed for a French Ligue 1 side Caen.
http://www.prestnaija.com/yaya-toure-signs-french-outfit/

Brazil world cup legend Rivaldo has warned every Nigerian and other sports fans to stay away from Brazil because of the level of crime in the country.


The world cup winner disclosed this on his Instagram page, Brazil will be hosting the world at the Olympics in August, Nigeria and the rest of the world will join in the Games of the XXXI Olympiad but the Brazilian World Cup winner and Legend is far from excited, the former FC Barcelona player warned those who has nurtured plans to visit the South American country for the games in Rio 2016 Olympic Games to kindly stay away from the country (Brazil) for their own safety.

Rivaldo lamented on Sunday haven heard about the murder of a 17 year old girl.

“Things on the daily are getting uglier here in Brazil,” Rivaldo had posted on his Instagram page… “I would want to advise everyone who has made plans to visit Brazil during the Olympics in Rio – to please stay back at home. You will be putting your life in danger and you will be at risk here.”

Rivaldo then added, “This is without even speaking about the state of public hospitals and all the Brazilian political mess. Only God can save the us and change the Brazil situation.”

Olympics begins in Rio on the 5th of August, 2016, despite the violence in the country, and the water issues, including the Zika virus scare and everything other vices that could cause harm.
http://www.prestnaija.com/rivaldo-warns-nigerians-others-stay-home-olympics/

Its Mavin Record 4th Anniversary Today

When the journey started nobody ever thought it will ever get this big, nobody believed they will have outstanding artists that will win awards.
http://www.prestnaija.com/mavin-record-4th-anniversary-today/

The Gospel On a sunday morning is worth more than a million, soul winning pays
http://www.prestnaija.com/sunday-message-wonders-soul-winning-part4/

Breaking - Teebillz tested negetive to drugs, who knows, she may be lying against the guy
http://www.prestnaija.com/teebillz-cleared-hard-drugs-testing-negative-cocaine-drug-related-substance/

Kelechi Iheanacho has grown into a striker that any team will want to have in there ranks, i think he deserve a pay rise but not $200k
http://www.prestnaija.com/city-striker-iheanacho-demands-200000-per-week/

Word Focus :- Covenant Day Of Recovery

This weeks Sunday Message will focus on FAITH, Yes faith, see its definition and how faith works. Faith is not a philosophical assertion, but it is a mystery of the kingdom, it is not just a believe system, it us a spiritual weapons if warfare.
http://www.prestnaija.com/sunday-message-engaging-wonders-faith-prt-1a/

In an exclusive interview Tiwa made some mind blowing revelations where she accused her husband of drug addiction and debtedness, in the interview with Azuka Ogujiuba of ThisDay Editor, and was obtained exclusively by Pulse TV, Tiwa stated the following....
http://www.prestnaija.com/teebillz-cocaine-addict-investigated-efcc-tiwa-savage-interview/

Falz disclosed to his fans letting them to know that both he and Simi are yet to start dating as against what most people think.

The speculations on their alleged relationship was fueled by the nature of event surrounding the relationship between rapper, Falz tha badt guy and the singer Simi, the two artistes have worked closely together on some ‘A list’ songs and they are very popular in Nigeria’s music industry

http://www.prestnaija.com/falz-badt-guy-discloses-relationship-simi/

So finally he'll be leaving badoo, I hope his fire never quenches, Keep the flag flaying bro @Lil Kesh
http://www.prestnaija.com/olamide-finally-reponds-lil-kesh-departure-ybnl/

Islamic Group Begs FG To List Fulani Herdsmen As Terrorists
http://www.prestnaija.com/islamic-group-begs-fg-list-fulani-herdsmen-terrorists/

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Paul's brother, Florentin would be a happy man since his vote for Messi and Ronaldo ended up getting one the award while the other came second.

Pogba senior would have at least his brother ass his third pick but the Guinea international voted for Manuel Neuer instead while Paul was left out in the running for the award.

Guess this is something that even Pogba will ponder upon.
http://www.prestnaija.com/paul-pogba-refused-a-ballon-dor-vote-by-brother/

Marriage is the first institution God inaugurated, One must be carefull before going into it........
http://www.prestnaija.com/sunday-message-gods-word-in-marriage/

with the recent event at BenBruce At 60 I think these two guys are far from friends even as most of us may even think, Don jazzy turns dbanj down again http://www.prestnaija.com/don-jazzy-rejects-offer-to-perform-with-dbanj/

Apparently one thing these guys cant stop doing is keepin there pants on, Samuel Eto has been accused by an Olosho he used to have, now she wants to release a book titled REVENGE PORN......... Thats why Drogba is better than these guys all, no controversy anywhere http://www.prestnaija.com/samuel-etoo-s-former-mistress-plans-to-release-a-revenge-prn-book/

Even tho their split is non of my business, this guys should stop shaming there family name biko, every thing is meant for social media, every small thing they will bring it here, Mr and Mrs Okoye wont be happy about this. Bring the family name to the ground. And this rubbish started wen there mum past on God rest her soul........http://www.prestnaija.com/jude-okoye-leaves-p-square-management/

LOL! Everybody should just relax, Pquare are not splitting, remember they did the same thing in 2014 and then came out with an album Double trouble, just watch out, some mysterious person will come now and settle them and they will release a new album ........... thats Nigeria entertainment for you, you must hype your album before you release it..... http://www.prestnaija.com/psquare-and-jude-okoye-spits-fire-on-twitter/

If no evidence has been found on these couple they should just let them be, today you arrest the wife NOTHING tomorrow you arrest the husband NOTHING still, am tired of it already, Let the madueke rest biko http://www.prestnaija.com/alison-amaechina-madueke-arrested-by-efcc/

Konji na truly bastard, so the said woman wants to kill the boy just for her satisfaction ..........SMH these are the kind of news the hubby will hear and will ask for divorce http://www.prestnaija.com/married-woman-on-the-run-over-sx-drugs-overdose/

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