sainty2k3:

You can still give your analysis let's here or open another thread and explain better ,I will be happy to see that from you.

joyandfaith:


Tell Dr Abiessuku of Chemical pathology you have poor knowledge of hemoglobininopathies. Tell Dr Osho P.O that you were sleeping in class during lectures. Partial knowledge is your problem. Pls, do not say AA and AS can produce SS in any conference. if you do, u get know in ur head. so, if chromatographymedical or pcr is used, AA and AS will still produce SS. nonsense.

joyandfaith:


because op thinks he is talking to health workers or medical researchers. many people here cannot analyze it logically. we could have helped him to analyze if topic is changed.

innosaint27:








There are many discrepancies these days with Lab results due to quackery, but that is being checked by the relevant body concerned.

There are many individuals who assume their genotype and blood group. You ask them & they tell u " i am genotype AA and blood group O+" without ever going for the test. There are others who go for the test but end up in the hands a quacks. It is highly recommended that such tests be done in Teaching Hospitals or General Hospitals. Not private facilities pls.

A father and mother who both claims to be AA but produces an offspring who is AS or SS will have lots of questions to ask. But, before they do, let them be honest with themselves and go for the test in a recommended facility. If they have done so before, let them have it repeated in that facility and elsewhere again. MUST BE A GOVT HOSPITAL. Likewise that of their offspring.

At the end of the day, the discrepancy will be uncovered.

Most times, you will discover that either of the parents had never been tested or was tested but in a quack facility where wrong result was issued.

jnrremedy:

I never said anywhere that AS and SS will produce SS u now that
pls read all my post and point to where I said that.
that is one thing I don't like about doctors ( i have a lot of them as freinds) they believe they know it all that nobody can know more than them

jnrremedy:

I never said anywhere that AS and SS will produce SS u now that
pls read all my post and point to where I said that.
that is one thing I don't like about doctors ( i have a lot of them as freinds) they believe they know it all that nobody can know more than them

sainty2k3:

That's the issue, how many people in Nigeria use genetic studies or HPLC to find out there HB genotype . all people do is HB electrophoresis and say they are AA ,AS or SS and those are the people my topic refers to , note that in the topic I didn't say that's there genotype. that's the phenotype, they wrongly refer to it as genotype.

innosaint27:








It is important we disseminate info with valid facts especially on sensitive topics like this.

Hb electrophoresis remains the conventional diagnostic investigation for genotyping all over the world.

There are other advanced techniques like PCR and Isoelectric focusing for confirm genotypes but these investigations are very expensive & most hospitals including tertiary health facilities don't have them.

The thalassamias are known to rarely occur in the black population. The prevalence is very low.


I accepted the OP to tell us, what Thalassamias are, how they are inherited, diagnosis, clinical presentation of patients, laboratory findings in such patients etc. And not confuse the public by telling us it is possible for AA + AS = SS.

That is a fallacy & a scientific blunder!

joyandfaith:


but you are defending op misleading topics. no doctor knows it all. and you do not generalize ok.

sainty2k3:

Don't mind him. From his latest post I can see he has understood the analysis u made.

sainty2k3:


U mean all over the word HB electrophoresis is the conventional way for genotype testing? No, at least not alone, people combine with HPLC, FBC and blood film findings , other screening test to confirm the genotype if molecular studies will not be done

joyandfaith:


ur topic is wrong.

joyandfaith:


which analysis? what I know. it is topic that drags us to this level. it is misleading. see my first 2 posts.

sainty2k3:

Don't mind him. From his latest post I can see he has understood the analysis u made.

Syphonn:


can u help us cross it so we can see where the ss comes from when on parent is AA, ITS UNBELIEVABLE

sainty2k3:

You probably will not get it wrong of you do HPLC with HB electrophoresis or your electrophoresis can also quantify, but if not genotype Sbeta thal . will have an S only band on electrophoresis while A beta thal will have an A only band on electrophoresis which can be misinterpreted as SS and AA respectively

sainty2k3:

How many people of knowledge in the field of haematology do u know and if you know any have you tried to ask them

joyandfaith:


so are looking for analyzer because you did not understand d journal.understand textbook first before you read journal.

funmisticqueen:
5 consultants of which 3 are professors, eight senior registrars, need i say more, i would have asked them but maybe during the week.

sainty2k3:
, did the topic say genotype AA, can you see it 'AA' . what do you want the topic to look like, just responding to you cos I could sense you are a pathologist or pathologist in training. I think you need to be more explorative and open to learning new things . bye

naijaking1:


If you electrophorese valine and lysine will they come out the same? If you correctly answered no, then there is no way a good technologist will come to my office to tell me that the B-thal and Beta or alpha-thal are now migrating to the same spot on the plate. Unless your reagents are bad.
And, I think this is where you got confused. The two hemoglobinopathies don't have the same electrophoretic pattern in a good lab! So that patient you had classified as AA, or AS might have been wrong in the first place.

nelszx:


Thalassemia wouldn't show anything (A or SS) on Hb electrophoresis. You might see HbH (Barts Haemoglobin) but definitely not A band or SS , it's best diagnosed using CBC (HB and the RBC indices) with Ferritin level

sainty2k3:

You don't need to be insultive as you have been , just take your time, one day in your practice this fact will be useful to you

sgtponzihater1:


Why do we have these kind of dudes pls?, they don't know and avoid learning. Use the internet. Type thalassemia in Nigeria, tell me what u get. Shit!

sainty2k3:

Alright we can talk about it when you have asked them.
I hears it first directly from one of the country's greatest professor of haematology, and I have also verified it, seen this kind of scenarios

joyandfaith:


which fact? I do not ur info. if you want 20 journals on misdiagnosis in hemoglobin testing, I shall provide the links. it is ur annoying argument that take us here. initially, you were told that information is misleading but u refused to listen.

joyandfaith:


the professor will deny u. u did not understand what professor said.

sainty2k3:

In the concept of thalassemia, one or more of the globin gene is deleted or mutated , so what is absent can not move, and yes, HbSS and HbS- b thal, will look similar on alkaline media electrophoresis

naijaking1:


Ok, once again, the difference is valine and lysine.
If you don't know already, the genetic pair of the beta globulin chain of the hemoglobin is mutated, and they transcribe these amino acids instead of glutamic acid. Presence of valine or lysine changes the PH and pk values of the new hemoglobin solution so they sickle and deform in shape-a condition that makes the spleen pit them out of the circulation with resulting anemia, vaso-occlussive conditions, etc.
The electrophoresis picks up the transcribed elements, while gene splicing is what you need to make the diagnosis at the sequencing level.

sainty2k3:


Someone got it crossed here

https://www.stjude.org/treatment/disease/sickle-cell-disease/diagnosing-sickle-cell/beta-thalassemia-trait.html