It's a man's world, take it or leave it

NOPE

omo

omo

Nice one

Update

Lol

2c. Madam Nneka is a drug discovery scientist who needs to determine the therapeutic activity of a newly discovered drug candidate in vitro. To achieve this, she needs to first prepare different concentrations of the candidate drug. If Madam Nneka has 100 ml of 2 g/ml stock solution of the drug candidate, explain (each separately) how she would prepare 10 ml each of 500 pg/ml, 250 ug/ml and 125 ug/ml of the drug candidate.

To prepare 10 ml of 500 pg/ml of the drug candidate, Madam Nneka would need to dilute the stock solution by a factor of 10,000. This can be done by adding 9.9 ml of water to 0.1 ml of the stock solution.

To prepare 10 ml of 250 ug/ml of the drug candidate, Madam Nneka would need to dilute the stock solution by a factor of 8,000. This can be done by adding 9.9 ml of water to 0.1 ml of the stock solution.

To prepare 10 ml of 125 ug/ml of the drug candidate, Madam Nneka would need to dilute the stock solution by a factor of 16,000. This can be done by adding 9.9 ml of water to 0.01 ml of the stock solution.

3a. Explain how the blood plasma is buffered by the bicarbonate system.

The bicarbonate system is a buffer system that helps to maintain the pH of blood plasma. The system consists of bicarbonate ions (HCO3-) and carbonic acid (H2CO3). When the pH of blood plasma decreases, the bicarbonate ions react with hydrogen ions (H+) to form carbonic acid. This reaction helps to buffer the pH of blood plasma and prevent it from becoming too acidic.

3b. Derive the Henderson-Hasselbalch equation.

The Henderson-Hasselbalch equation is a mathematical equation that describes the relationship between the pH of a buffer solution and the concentrations of the acid and base components of the buffer. The equation is as follows:

pH = pKa + log(A-/HA)

Where:

* pH is the pH of the buffer solution
* pKa is the pKa of the acid component of the buffer
* A- is the concentration of the anion of the acid component of the buffer
* HA is the concentration of the acid component of the buffer

3c. If the plasma pH is 7.4 and the plasma concentration of HCO3- is 15 mM, what is the plasma concentration of H2CO3? (Note pKa of carbonic acid is 6.1)

The plasma concentration of H2CO3 can be calculated using the Henderson-Hasselbalch equation. The equation is as follows:

pH = pKa + log(A-/HA)

7.4 = 6.1 + log(15/HA)

1.3 = log(15/HA)

10.1 = 15/HA

HA = 1.5 mM

Therefore, the plasma concentration of H2CO3 is 1.5 mM.

4a. Explain the basis for the buffering activities of buffer solutions?

Buffer solutions work by absorbing or releasing hydrogen ions (H+) in order to maintain the pH of a solution. The buffering capacity of a buffer solution is determined by the concentrations of the acid and base components of the buffer. The greater the concentrations of the acid and base components, the greater the buffering capacity of the buffer solution.

4b. List five important criteria to be considered in choosing a buffer for a biochemical analysis

Five important criteria to be considered in choosing a buffer for a biochemical analysis are:

* The pH of the buffer should be within the range of the pH of the biochemical reaction that is being studied.
* The buffer should have a high buffering capacity.
* The buffer should be chemically stable.
* The buffer should not interfere with the biochemical reaction that is being studied.
* The buffer should be inexpensive and easy to obtain.

4c. Given 500 ml each 0.5M solution of citric acid and 1M solution of sodium citrate, describe how you will prepare 100 ml of 0.1M citrate buffer with a pH of 4.5 (The pKas of citric acid are:3.1,4.7 and 6.4).

To prepare 100 ml of 0.1M

Pop OS

Okporiaku:

U mean your kuda card worked on Google play store
If yes, whats the rate

Not on play store, on Google Cloud Platform

I used my virtual kuda card yesterday and it worked, I was very surprised

Mother Mary

Likoya:
Shut up, you gay man.... It's true

Lol, you just be assigning names to people

Lol, people just sit and spew some sh*t

Omo

Omo

Omo

Omo. Ah, my brother! You don come to the right place, Nairaland, where we dey drop knowledge like pure water for your thirsting career quest. Ehen, make I yarn you the truth as e be. The best career, na the one wey you sabi do wey go put food for your table and enough jara for your pocket. If you think say I go just come here dey pour grammar like Wole Soyinka, you go wait tire. No be joke oh!

Make I tell you one secret: for this 21st century wey we dey so, tech skills na the key to unlock doors wey dey lead to success. E no be like that time wey our forefathers dey hunt bush meat for jungle. If you never dey sharpen your tech skills, my brother, you go soon become oyibo for your village. The world don change oh! No be only "Iyawo mi" dem dey wire for village, dem dey wire Bitcoin too.

So, if you wan shine for this life, make you hustle to learn tech skills. No be only programming oh! Anything wey get to do with tech, whether na web design, cybersecurity, digital marketing, or even video editing, just pick one wey sweet your belle and run with am. Na so dem dey say, "The sky na your stepping stone." But if you like, carry stepping ladder climb the sky, na your own wahala be that.

Leveraging freelancing na another game changer for career. See, no be only your papa office you fit work, you fit use your talent sell am to the highest bidder for freelancing platforms like Upwork, Fiverr, and the rest of them. Just make sure say you no go fall victim to those clients wey dey like to pay with expo. Know your worth and collect your money complete before you finish work. No dey form, "I be charitable organization."

But wait oh, make I tell you something funny. For this Naija, e get some people wey go dey call themselves "Yahoo Yahoo boys" and "G-boys" and dey think say na career. Abeg, if you see any of them, just bone face, commot eye, commot ear. No follow them do meeting, unless you want make EFCC use your name swear for court. Hustle legit, my brother. Yahoo no be career, na express ticket to Kirikiri prison.

So, make you find your passion, build your tech skills, leverage freelancing, and hustle legit. I dey tell you, the world na your village; conquer am! If you like, take this advice, if you like, leave am. Na only you fit write your own success story. No forget say, "Person wey wan fly like eagle, no suppose follow pigeons dey waka."

Wetin I talk? E do, abi I suppose add salt and Maggi join? Make I drop my pen here before I begin preach. May the hustle be with you, my guy!


https://chat.openai.com/share/9229ee49-cb71-4273-868d-b3a16ac658c8

Nice one

Omo

Omo

Omo

Omo

'I hope this email finds you well' Chatgpt spotted

No phone number on profile?

Nope

Normally

Omo

😂 😂 😂

omo 💔

tensazangetsu20:

Work visa for countries under NAFTA. Mexicans and Canadians can work in the USA using the TN visa due to being in the north American free trade agreement

Okay thanks 👍